Proteinases with various substrate specificities in structural studies of yeast cell walls
Kalebina, T.S.; Nurminskaia, M.V.; Zhang, S.; Chertov, O.I.; Rudenskaia, G.N.; Stepanov, V.M.; Kulaev, I.S.
Bioorganicheskaia Khimiia 20(6): 627-634
1994
ISSN/ISBN: 0132-3423 PMID: 7945458 Document Number: 428178
Different proteins are revealed in cell wall of yeast cells Candida utilis by means of specific proteolysis with subtilisins TV and 72, trypsin and purified collagenase of Clostridium histolyticum. Some of them were characterized by resistance to trypsin and sensitivity to subtilisin TV. In young cells this group is represented essentially by a protein of 33 kD, which appears to be one of the structural proteins, binding fibrillae of carbohydrate. Other proteins proved to be sensitive to both trypsin and subtilisin. Among these proteins a protein with mol. mass 80 kD was revealed; its sensitivity to extremely specific hydrolysis by bacterial collagenase suggests it to contain amino acid sequences characteristic for collagens of higher eukaryotes.