Non-specific agglutination of Mycoplasma gallisepticum
Bradbury, J.M.; Jordan, F.T.
Veterinary Record 92(22): 591-592
1973
ISSN/ISBN: 0042-4900 PMID: 4730113 Document Number: 64854
Antigens from three strains of M. gallisepticum (A514, S6 and X95), prepared from globulin-free and serum supplemented broth, were examined for non-specific agglutination reactions with three groups of chicken sera. All the antigens except A514 prepared in globulin free broth were susceptible to agglutination by serum from the three groups. However, when incubation was continued for 7 days in globulin-free broth the A514 antigen was highly susceptible to non-specific agglutination. S6 antigens prepared after 7 days incubation in globulin-free broth gave more non-specific reactions than the control antigens from serum supplemented broth. Evidence suggests that elimination of adsorbed globulins does not necessarily yield a product of increased antigenic specificity, and that these globulins may play a minor role in non-specific agglutination reactions.