Virus-like particles in an equine sarcoid cell line
England, J.J.; Watson, R.E.; Larson, K.A.
American Journal of Veterinary Research 34(12): 1601-1603
1973
ISSN/ISBN: 0002-9645 PMID: 4796998 Document Number: 63110
Electron microscopic examination of a cell line derived from an equine sarcoid revealed intracytoplasmic oncornavirus-like particles. Cells treated with idoxuridine-dimethyl sulphoxide (IDU-DMSO) showed a two- to four-fold increase in the number of particles as compared with untreated cells or cells treated with DMSO alone. The intracytoplasmic virus-like particles were surrounded by a double membrane and measured 80 to 100 nm in diameter. Particles were seen extracellularly or budding from the cell membrane into the extracellular space. These extracellular particles were 100 nm in diameter and contained an electron-lucent core 60 to 70 nm in diameter.