Apparent propagation of the equine infectious anemia virus in a mosquito (Culex pipiens quinquefasciatus Say) ovarian cell line
Breaud, T.P.; Steelman, C.D.; Roth, E.E.; Adams, W.V.
American Journal of Veterinary Research 37(9): 1069-1070
1976
ISSN/ISBN: 0002-9645 PMID: 183574 Document Number: 103134
Culex pipiens fatigans Wied. (pipiens quinquefasciatus auct.) occurs in massive numbers in Louisiana, where equine infectious anaemia occurs in particular areas. Several species of mosquitoes are stated to have been implicated in transmission of the virus in Japan. A tissue culture of ovarian cells of C. p. fatigans appeared to support the growth of the virus. Shetland ponies that were inoculated with the second, seventh, ninth and eleventh passages but not the third and fifth, showed clinical signs of the disease and became positive in tests 11, 19, 23 and 43 days after inoculation, respectively.