Application of pulse-field gel electrophoresis analysis in source-tracking of food-borne disease caused by Vibrio parahaemolyticus
Deng, Z-Ai.; Li, X-Quan.; Hu, Y-Shan.; Liu, J-Hua.; Zhang, X-Qiang.; Lin, Y-Wan.; Mo, Z-Yao.
Zhonghua Yu Fang Yi Xue Za Zhi 42(1): 36-38
2008
ISSN/ISBN: 0253-9624 PMID: 18512325 Document Number: 617577
Objective To apply pulse-field gel electrophoresis analysis(PFGE) in analysing a case of food poisoning caused by Vibrio parahaemolyticus. Methods PFGE using restriction enzyme Not I was employed in molecular subtyping of thirty strains of V. parahaemolyticus isolated from a case of food poisoning in Guangzhou city and PFGE patterns were analyzed by using BioNumerics Version 4.0 software to perform cluster analysis. Pattern profiles were compared by using the Dice coefficient and unweighted pair group method with arithmetic averages (UPGMA). Results Thirty strains were of the same type of pulsotype. Conclusions Molecular subtyping by PFGE might disclose the epidemiological relationships of the strains from humans food and the environment, giving a strong molecular epidemiological evidence and a support for the source-tracking of outbreak events.