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Accurate evaluation of the sizes of DNA fragments (from 30 to 4700 kb) in pulse field gel electrophoresis

Crété, N.; Delabar, J.M.; Sinet, P.M.; Créau-Goldberg, N.

Biotechniques 11(6): 711

1991

ISSN/ISBN: 0736-6205
PMID: 1667081
Document Number: 383903
Construction of long-range genomic maps by pulse field gel electrophoresis requires optimum resolution of large DNA fragments. Using the transverse-alternating field electrophoresis system, we describe a method to accurately evaluate the sizes of fragments generated by rare-cutter digestions within the 30-4700-kb range. A protocol generating large (greater than 1000 kb) molecules by partial digestion is also reported.

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