Electrophoresis of DNA fragments renatured and cross-linked with psoralen: kinetic analysis of DNA photocross-linking
Gonikberg, E.M.; Andreev, V.M.
Molekuliarnaia Biologiia 20(4): 1039-1047
1986
ISSN/ISBN: 0026-8984 PMID: 2945091 Document Number: 279708
The kinetics of photoreactions between DNA and psoralen was analysed. A method is described for determining the dependence of the average number of cross-links per base-pair (n) on the fluence of UV-irradiation. This calibration curve allows to specify the conditions of irradiation necessary to obtain any desired value of n.
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