Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay
Tsang, V.C.; Wilson, B.C.; Maddison, S.E.
Clinical Chemistry 26(9): 1255-1260
1980
ISSN/ISBN: 0009-9147 PMID: 6772340 Document Number: 163427
We describe a single-cuvette enzyme-linked immunosorbent assay (ELISA) based on enzymic rate kinetics. This kinetic assay can yield linear quantitative data on immunoglobin concentrations. Optimum assay conditions, component concentrations, reaction intervals, and pH are described. Assay linearity and sensitivity are demonstrated in systems involving immunoglobulin G and antibodies to it, and with pooled sera from monkeys infected with Schistosoma mansoni.