Enzyme-linked immunosorbent assay for deoxynivalenol in corn and wheat
Xu, Y.C.; Zhang, G.S.; Chu, F.S.
Journal - Association of Official Analytical Chemists 71(5): 945-949
1988
ISSN/ISBN: 0004-5756 PMID: 3235414 Document Number: 314224
Using antibody against deoxynivalenol (DON) triacetate (Tri-Ac-DON), direct and indirect ELISAs for DON in maize and wheat were developed. In both assays, DON was extracted from the sample with acetonitrile-water, reacted with acetic anhydride to form Tri-Ac-DON and diluted in phosphate buffer for analysis. Direct ELISA was found to be the more sensitive procedure. Fewer interferences were evidenced and the assay was less time consuming than indirect ELISA. For direct ELISA, recovery of 10-1000 p.p.b. DON added to maize and wheat was 100 and 102.1%, respectively. For indirect ELISA, overall recovery of 10-1000 p.p.b. DON added to wheat was 121.5%; in the higher concn range (500-1000 p.p.b.), recovery was 105%. The min. detection level for DON was approx. 10 p.p.b. Analysis of 7 naturally contaminated samples for DON showed that the ELISA results agreed well with those obtained by radioimmunoassay and thin-layer chromatography.