Thin layer chromatographic determination of deoxynivalenol in wheat and corn
Trucksess, M.W.; Nesheim, S.; Eppley, R.M.
Journal - Association of Official Analytical Chemists 67(1): 40-43
1984
ISSN/ISBN: 0004-5756 PMID: 6698929 Document Number: 224225
A thin layer chromatographic (TLC) method for determining deoxynivalenol (DON) in corn and wheat was developed. DON is extracted from the grain with acetonitrile-water (84 + 16) and filtered through a column of mixed alumina-charcoal-Celite (0.5 g + 0.7 g + 0.3 g). The solvent is evaporated on a steam bath. Ethyl acetate is added to the residue and heated to dissolve DON. After cooling, the residue is transferred to a vial with additional ethyl acetate and is dissolved in CHCl3-acetonitrile (4 + 1) for TLC on an AlCl3-impregnated silica gel plate with CHCl3-acetone-isopropanol (8 + 1 + 1). The plate is heated in a 120 degrees C oven for 7 min; a blue fluorescent spot is produced under longwave ultraviolet light. DON is quantitated visually and/or fluorodensitometrically by comparison with reference standards. The minimum detectable amount of DON is ca 20 ng/spot. The limit of DON determination is ca 40 ng/g for wheat and 100 ng/g for corn. Recoveries of DON added to wheat and corn at 100, 500, and 1000 ng/g levels were 85, 93, and 88% and 77, 80, and 80%, respectively.