High-performance thin-layer chromatographic determination of rabeprazole sodium and domperidone in combined dosage form
Gandhi, S.V.; Khan, S.I.; Jadhav, R.T.; Jadhav, S.S.; Jadhav, G.A.
Journal - Association of Official Analytical Chemists 92(4): 1064-1067
2009
ISSN/ISBN: 0004-5756 PMID: 19714973 Document Number: 635448
A new, simple, high-performance thin-layer chromatographic method for determination of rabeprazole sodium (RAB) and domperidone (DOM) in combined tablet dosage form has been developed and validated. The mobile phase was toluene-acetone-methanol (4.5 + 4.5 + 0.5, v/v/v) with UV detection at 285 nm. The retention factors for RAB and DOM were found to be 0.53 +/- 0.12 and 0.32 +/- 0.20. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 50-800 ng/band for both RAB and DOM. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.