Evaluation of an Automated Capillary Electrophoresis System for Serum Protein Electrophoresis with the Determination of Gender-Specific Reference Values
Lichtinghagen, R.; Pietsch, D.; Brand, K.
Clinical Laboratory 56(3-4): 119-126
2010
ISSN/ISBN: 1433-6510 PMID: 20476643 Document Number: 12501
In this report we summarize evaluation data of an automated capillary zone electrophoresis ( Capillarys II, Sebia , France) in comparison to a conventional cellulose acetate electrophoresis system ( Hite320, Olympus , Germany). Serum samples from 428 blood donors were analysed to evaluate gender-specific reference intervals, which revealed significant percentage differences between male and female. In contrast to conventional electrophoresis methods all lipoproteins co-migrate with the albumin fraction using capillary electrophoresis. Comparisons between both separation methods and quantitative determinations of albumin/total protein show a better correlation between the albumin fraction determined by capillary electrophoresis and the quantitative determinations. A transversal evaluation of serum protein fractions should be performed within one day due to the combination of effects based on serum sample instability and method imprecision.
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