Determination of histamine in trypsine by capillary zone electrophoresis--a possible alternative to the bioassay?
Miethe, G.; Surmann, J.P.
Die Pharmazie 56(6): 462-464
2001
ISSN/ISBN: 0031-7144 PMID: 11446165 Document Number: 532611
A rapid capillary electrophoresis separation was developed in order to separate histamine from trypsine. A calibration curve with a determination limit of 1.0 microgram/ml histamine in 1 mg/ml trypsine was obtained. The method is suitable for the detection of small amounts of histamine contamination in trypsine with regard to the standards of the European Pharmacopoeia.
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