Quantitative analysis of the nucleosides in Cordyceps sinensis with capillary zone electrophoresis
Hou, X-Rong.; Luan, L-Jun.; Cheng, Y-Yu.
Zhongguo Zhong Yao Za Zhi 30(6): 447-449
2005
ISSN/ISBN: 1001-5302 PMID: 15810452 Document Number: 590947
Objective: To establish a quantitative analysis method for analyzing the nucleosides in Cordyceps sinensis with capillary electrophoresis, and compare the difference between natural and the cultured C. mycelia. Method: Capillary zone electrophoresis method was employed to quantitate the adenosine, uridine, guanosine and inosine in C. sinensis, with 0.25 mg.L-1 boric acid-sodium hydroxide buffer, pH 9.5. The working voltage was 20 W, the temperature was 25 degrees C, and the detection wavelength was 260 nm. Result: With the capillary zone electrophoresis method, the average recovery of the above 4 nucleosides was 98.9%, 95.1%, 97.8% and 98.8% respectively, with the RSD 0.4%, 1.7%, 1.3% and 5.0%. There was no adenosine in natural C. sinensis and no inosine in the cultured C. mycelia detected. Conclusion: This method can be used to determine the adenosine, uridine, guanosine and inosine in C. sinensis. The nucleosides in C. sinensis produced from Qinghai province and cultured C. mycelia are obviously different.