Determination of two saponins in Tupistra chinensis rhizomes by RP-HPLC
Zhou, Y.; Zou, K.; Yu, L.-l.; Qin, S.-j.; Xu, L.-l.; Liu, C.
Zhongguo Zhong Yao Za Zhi 33(22): 2647-2649
2008
ISSN/ISBN: 1001-5302 PMID: 19216163 Document Number: 615614
To develog an HPLC method determination of two new saponins in the dried rhizomes of Tupistra chinensis. The analysis was performed on YMC-Pack ODS-AQ (4.6 mm x 250 mm, 5 microm) eluted with of acetonitrile and waterin gradient mode. The conentration of acetonitrile in the mobile phase changes from 10% to 100% within 85 minutes. The detection wavelength was set at 203 nm. The flow rate was 1.00 mL x min(-1) and column temperature was set at 30 degrees C. The linear relationships of two authentic saponins were determined within the range from 7.55 microg to 60.40 microg (r=0.9996) and 8.00 microg to 48.00 microg (r=0.9996), respectively. Using the method above, the contents of two saponins were determined as 0.261% and 0.242%, with the recoveries as 98.6% and 98.3% with RSD 1.7% and 1.7%, respectively. A convenient and reliable method was developed to determine the content of two saponins in the dried rhizomes of Tupistra chinensis.