Characteristics of passive Ca2+ -transport from cells of the myometrium sarcoplasmic reticulum in rats

Danylovych, I.V.

Fiziolohichnyi Zhurnal 53(1): 55-61

2007


ISSN/ISBN: 2522-9028
PMID: 17500203
Document Number: 608932
With use of model ofpermeabilizated myocytes from rat uterus and 45Ca2+ it is shown, that passive Ca2+ transport from sarcoplasmic reticulum (cation has been preliminary accumulated in ATP-dependent process) is sensitive to 2 mM caffeine, 0,1 mM lidocaine, and also to exogenous Ca2+ (it was activated at increase in concentration Ca2+ from 10(-7) up to 10(-4) M and was suppressed at 10(-3)) and pH level (was stimulated by alkalization of medium), along with significant suppression of Mg2+ (0.2 - 4 mM). The analysis of the results received allows us to assume that the model used by us is adequate for studying processes of Ca2+ release from sarcoplasmic reticulum, mediated by ryanodine receptor channels.

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