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Blockers of the uncoupling action of caffeine on the Ca2+-transport function of sarcoplasmic reticulum membranes

Ritov, V.B.

Biulleten' Eksperimental'noi Biologii i Meditsiny 99(4): 445-448

1985

ISSN/ISBN: 0365-9615
PMID: 2580576
Document Number: 253759
The influence of caffeine on the efficiency of Ca2+ transport in the presence of oxalate by different fractions of sarcoplasmic reticulum (SR) membranes from rabbit skeletal muscle was studied. It was shown that caffeine (5 mM) decreases 4-fold the value of the Ca/ATP ratio in terminal cisterns of the SR without having any appreciable influence on the efficiency of Ca2+ transport by the light fraction of the SR. The uncoupling effect of caffeine is completely blocked by ruthenium red and by the local anesthetics, tetracaine, procaine, and benzocaine.

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