Effects of Arg-Gly-Asp-Ser on Ca2+ transport of myocardial sarcoplasmic reticulum in rat septic shock
Ji, Y.; Zhao, M.; Qi, Y.; Dong, L.W.; Wu, L.L.; Peng, S.Q.; Su, J.Y.
Zhongguo Yao Li Xue Bao 17(2): 129-132
1996
ISSN/ISBN: 0253-9756 PMID: 9772661 Document Number: 459067
AIM: To study the effects of Arg-Gly-Asp-Ser (RGDS), a synthetic short peptide of fibrinogen degradation, on the Ca-2+ transport function of cardiac sarcoplasmic reticulum in rat septic shock. METHODS: RGDS 5 mu-mol cntdot kg-1 was injected iv at 4 h and 14 h after cecal ligation and puncture (CLP) operation on rats. Highly purified membrane of sarcoplasmic reticulum (SR) was prepared from rat hearts. Assays were made of ATP- dependent Ca-2+ uptake by cardiac SR and (3H) ryanodine binding to SR. RESULTS: The initial rate and the capacity of SR Ca-2+ uptake were increased by 104% ( P lt 0.01) and 12% (P lt 0.05), respectively, paralleled by an increase in Ca-2+ -ATPase activity and a decrease in calcium accumulation of myocardium of septic rats, whereas the B-max and K-d values of Ca-2+ activated (3H) ryanodine binding to SR were unaffected after RGDS administration. CONCLUSIONS: The results indicated that RGDS have cardioprotective effects of maintaining Ca-2+ homeostasis of cardiac myocytes by enhancing SR Ca-2+ uptake in rat septic shock.