Determination of triptolide in Tripterygium total terpenoids tablets by HPLC
Pu, C-hai.; He, M-fang.; Zhanag, X-qian.; Shen, J.
Zhongguo Zhong Yao Za Zhi 30(4): 266-267
2005
ISSN/ISBN: 1001-5302 PMID: 15724401 Document Number: 587022
Objective: To esdevelop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets. Method: A Lichrospher CN column was used with wethanol and water for gradient elution. The detection wavelength was set at 255 nm. Result: The linear relationship of the concentrations and peak areas was good in range of 0.742-59. 4 mug . mL-1 (r = 0. 999 8). The average recovery was 99.2%, RSD% = 1.7%. Conclusion: The method is simple, rapid and accurate and can be used for quality control of the tablets.