Glycyrrhizinic Acid Reference Standard (Control 031) of National Institute of Health Sciences
Koide, T.; Murakami, M.; Morita, Y.; Saito, H.; Tanimoto, T.
Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo Hokoku 2003(121): 54-56
2003
ISSN/ISBN: 1343-4292 PMID: 14740407 Document Number: 560491
The raw material of glycyrrhizinic acid was examined for preparation of the "Glycyrrhizinic Acid Reference Standard". The analytical data obtained were: UV spectrum: lambda max, 251 nm; and specific absorbance (E(1 cm)1%) in ethanol at 251 nm, 146.4; IR spectrum, specific absorptions of raw material were consistent with those of Standard (Control 0221). Also, thin-layer chromatography, no impurity was detected; high-performance liquid chromatography, several impurities were detected. The amount of each impurity was estimated at less than 0.2% and total amount of impurities was less than 0.2%. Based on the above results, the candidate material was authorized as the Glycyrrhizinic Acid Reference Standard (Control 031) of the National Institute of Health Sciences.