The Cyanocobalamin Reference Standard (Control 931) of the National Institute of Health Sciences
Kitajima, A.; Yoshii, K.; Komatsu, H.; Ishimitsu, S.; Okada, S.
Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences 1994(112): 170-174
1994
ISSN/ISBN: 0077-4715 PMID: 8854923 Document Number: 431484
Raw cyanocobalamin material was tested for preparation of the "Cyanocobalamin Reference Standard (Control 931)". Analytical data obtained were as follows: loss on drying, 4.2%; infrared spectrum, the same as that of the JP Cyanocobalamin Reference Standard (Control 901); thin-layer chromatography, four impurities were detected; high-performance liquid chromatography (HPLC), seven to eight kinds of impurities were detected, but the total amount of impurities was only 1.0 +/- 0.52% (n = 5); assay results, 100.6% by spectrophotometry in the JP XII and 99.7% by HPLC. Based on the above findings, the raw material was authorized as the JP Cyanocobalamin Reference Standard (Control 931).