Determination of phospholipid in the peroxisomal membrane of rat liver by high performance liquid chromatography
Jiang, Q.F.; Luo, Z.S.; Li, X.F.; Jiang, L.L.
Se Pu 18(2): 142-144
2000
ISSN/ISBN: 1000-8713 PMID: 12541591 Document Number: 518843
A method was employed for the determination of phospholipid in membrane of peroxisomes by high performance liquid chromatography. The separation was performed on a mu-Porasil Si60 column(250 mm x 4.6 mm i.d.) by gradient elution. The phospholipids were detected at 205 nm. The limits of detection for cardiolipin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin were 12, 8, 23, 4, 23 and 7 ng respectively and the recoveries were between 84% and 103%. The linear relationship of calibration curves was good (r > 0.91). The phospholipids in peroxisomal membrane of 10 SD rat liver were determined and the results are satisfactory.