Determination of histamine in tomatoes by liquid chromatography/mass spectrometry
Bolygo, E.; Cooper, P.A.; Jessop, K.M.; Moffatt, F.
Journal - Association of Official Analytical Chemists 83(3): 543-548
2000
ISSN/ISBN: 0004-5756 PMID: 10868574 Document Number: 513452
The histamine level in tomato fruits and pastes was determined by 2 orthogonal techniques as a means of comparing accuracy. Close statistical agreement was found between assays for free histamine by capillary electrophoresis (with UV absorbance detection), and for the dansyl derivative by reversed-phase liquid chromatography (LC). Both techniques have adequate sensitivity for the analysis of endogenous histamine in tomatoes, but LC/electrospray tandem mass spectrometry was more sensitive by at least an order of magnitude, down to a level of 0.05 mg/kg.