Identification of a neutral protease in the intestine of Boophilus microplus with electrophoresis in polyacrylamide gels copolymerized with gelatin
Hernández Alvarez, H.M.; Mendiola Martínez, J.; Fernández-Calienes, A.; Valdéz, M.
Revista Cubana de Medicina Tropical 52(3): 165-169
2000
ISSN/ISBN: 0375-0760 PMID: 11826517 Document Number: 513362
A neutral activity of Boophilus microplus in the intestine was identified by electrophoresis in polyacrilamide gel copolymerized with gelatin. The maximum of activity was attained at pH 6.0. The highest specific activity at that pH was obtained with casein substrate. The disappearance of this activity was observed in both substrates after the addition of phenylmethylsulphonyl fluoride in the reaction mixtures. It was very interesting to find out an endopeptidase activity with these characteristics in the intestine, in spite of the fact that the digestive activity in ticks is intracellular at very acid pH, which does not occur in other insects.