Nonradioactive mRNA differential display in polyacrylamide mini-gels
Lee, S.H.; Shiao, Y.H.; Kasprzak, K.S.
Research Communications in Molecular Pathology and Pharmacology 106(1-2): 108-114
1999
ISSN/ISBN: 1078-0297 PMID: 11127802 Document Number: 506126
Nonradioactive methods in comparison to those using radionuclides are now becoming popular because of safety, environmental, and cost concerns. The modified technique described here is much faster, easier, and safer than the conventional procedure utilizing radioactive material and large-formatted polyacrylamide gel, or other nonradioactive DD techniques. Also, it maintains good reproducibility and specificity. This DD protocol offers a rapid and reliable nonradioactive method for comparing gene expression patterns in various research.