Electroelution of DNA and protein from polyacrylamide and agarose gels
Chang, T.; Tsai, I.H.; Chang, W.C.
Biochemistry International 15(4): 687-691
1987
ISSN/ISBN: 0158-5231 PMID: 3435534 Document Number: 291500
An electroelution method is described for the recovery of DNA and protein from agarose of polyacrylamide gels. The samples to be electroeluted are compartmentalized in a modified microcentrifuge tube fitted with dialysis membranes. This procedure is simple, rapid, inexpensive and efficient. Within 30 min to 2 hrs, the recovery of the sample is nearly quantitative. DNA fragments recovered can be directly subjected to DNA sequence analysis or enzymatic reactions after ethanol precipitation. Proteins can also be recovered after separation by acrylamide gel in the presence or absence of detergents and be ready for further analysis.