Semi-dry electroblotting of DNA and RNA from agarose and polyacrylamide gels
Trnovsky, J.
Biotechniques 13(5): 800-804
1992
ISSN/ISBN: 0736-6205 PMID: 1384572 Document Number: 395325
Semi-dry electroblotting of nucleic acids was originally of interest for its speed and convenience, but it had significant limitations in reliability of transfer. This work describes optimalization of the buffer systems and general conditions for use that enable the semi-dry method of nucleic acids transfer to yield quantitative, fast and reliable results.