Alternative to polyacrylamide gels improves the electrophoretic mobility shift assay
Vanek, P.G.; Fabian, S.J.; Fisher, C.L.; Chirikjian, J.G.; Collier, G.B.
Biotechniques 18(4): 704-706
1995
ISSN/ISBN: 0736-6205 PMID: 7598903 Document Number: 443295
In this paper we outline a simplified protocol for the electrophoretic mobility shift assay utilizing TreviGel 500, a nontoxic alternative to polyacrylamide. The TreviGel 500 matrix combines the strength and resolution of polyacrylamide with the simplicity and flexibility of agarose in the casting of gels. Therefore, this method provides a simple, rapid and nontoxic alternative to current protocols for the investigation of protein: DNA interactions.