Approaches to assessing dynamics of permeability and duration of thiaminase i action, administered parenterally to animals
Puzach, S.S.
Voprosy Meditsinskoi Khimii 41(6): 43-44
1995
ISSN/ISBN: 0042-8809 PMID: 8619302 Document Number: 443294
Two approaches and direct isotopic assay were employed to examine the time course of permeability and duration of action of tissue thiaminase I injected parenterally to albino mice. The injected enzyme was found to be detectable in renal and hepatic tissues 3 hours later. The action of the enzyme dermally injected in a dose of 1.2 IU/kg in the same tissues lasted 4 days. Experimental findings led to the conclusion that the thiamine degradation enzyme may be used in experimental biology in order to estimate the duration of its action and the time course of gross molecular permeability in the tissue of test animals.