A technique for collecting saliva from the cattle-tick Boophilus microplus (Canestrini, 1887) using chemical stimulation. Environmental and temporal influences on secretion yield
Bechara, G.H.; Szabó, M.P.; Machado, R.Z.; Rocha, U.F.
Brazilian Journal of Medical and Biological Research 21(3): 479-484
1988
ISSN/ISBN: 0100-879X PMID: 3228628 Document Number: 320130
Injection of a pilocarpine solution into the haemocoel of females of B. microplus through the respiratory spiracle induced the flow of clear saliva, collected from the mouthparts with a capillary tube. The time interval between the detachment of the tick from its host and chemical stimulation influenced the volume of saliva secreted; secretion was greater during the first 2 h after detachment. There was a positive correlation between salivary yield and both environmental temperature and relative humidity.