DNA-fingerprinting of representatives of Bovinae subfamilies using the telomere markers (TTAGGG) 4
Semenova, S.K.; Vasil'ev, V.A.; Steklenev, E.P.; Prosniak, M.I.; Ryskov, A.P.
Genetika 35(1): 101-104
1999
ISSN/ISBN: 0016-6758 PMID: 10330618 Document Number: 509753
The (TTAGGG)4 oligonucleotide homologous to telomeric tandem repeats of human chromosomes was used for the first time as a multilocus hybridization probe for the analysis of genome variability in the two genera (Bos and Bison) of the Bovinae subfamily. DNA profiles for cattle, banteng, aurochs, and bison were obtained. Hybridization spectra were represented by the discrete individual- and species-specific bands characterized by codominant inheritance. For comparison, DNA profiles of the same samples obtained using the bacteriophage M13 DNA probe are presented. The usefulness of the microsatellite examined for the testing of pedigrees, description of intra- and interbreed variability as well as for determining relationships and the origins of the species of the Bovinae subfamily is discussed.