Telomere length and gene expression of shelterin in CD3 (+) T cell of severe aplastic anemia patients
Wang, T.; Fu, R.; Wang, H.-q.; Qu, W.; Ruan, E.-b.; Wang, G.-j.; Liu, H.; Wu, Y.-h.; Wang, X.-m.; Song, J.; Xing, L.-m.; Guan, J.; Li, L.-j.; Liu, H.; Liu, C.-y.; Wang, Y.-h.; Jiang, H.-j.; Ding, K.; Dong, X.-f.; Wang, H.-l.; Zhang, T.; Shao, Z.-h.
Zhonghua Yi Xue Za Zhi 93(20): 1533-1536
2013
ISSN/ISBN: 0376-2491 PMID: 24028717 Document Number: 669856
To explore the changes in telomere length and gene expression of complex shelterin (composed of 6 core components: TRF1, TRF2, POT1, TIN2, TPP1 and RAP1) in severe aplastic anemia (SAA). Bone marrow samples were obtained from 20 SAA patients and 10 normal controls. CD3(+)T cells were sorted by immunomagnetic separation. Telomere length was tested by Southern blot and the gene expressions of TRF1, TRF2, POT1, TIN2, TPP1 and RAP1 were detected by reverse transcription-PCR(RT-PCR). Telomeres of CD3(+)T cells were found significantly shorter in SAA untreated ((4.4 ± 1.1) kb, n = 9) and recovering groups((5.8 ± 1.0) kb, n = 11) than control group ((9.2 ± 3.3) kb, P < 0.05). Telomere length of CD3(+)T cells shortened with TH/S decreasing (r = 0.564, P = 0.029). The mRNA expression of POT1 decreased in untreated SAA patients (0.16(0.02-0.29)) and over-expressed in recovering patients (1.17(0.82-1.86), P < 0.05). The mRNA expression of RAP1 was significantly higher in untreated patients (4.14 (1.93-6.92)) than that in recovering group (0.87 (0.30-1.73) ) and controls (0.62 (0.45-4.07) , both P < 0.05). Changes in telomere length and shelterin gene expression occur in CD3(+)T cells of SAA patients and may be correlated with disease severity.