Expression of lymphokines in CD8 (+) HLA-DR (+) T lymphocytes of patients with severe aplastic anemia
Liu, C.-Y.; Fu, R.; Wang, J.; Liang, Y.; Qu, W.; Ruan, E.-B.; Wang, H.-Q.; Li, L.-J.; Liu, H.; Wang, G.-J.; Liu, H.; Wu, Y.-H.; Wang, X.-M.; Song, J.; Xing, L.-M.; Guan, J.; Wang, H.-L.; Zhang, T.; Liu, X.; Shao, Z.-H.
Zhonghua Yi Xue Za Zhi 92(18): 1240-1243
2012
ISSN/ISBN: 0376-2491 PMID: 22883060 Document Number: 662870
To examine the expression of lymphokines damaging hematopoietic cells by CD8(+)HLA-DR(+) effector T cells in peripheral blood (PB) of the patients with severe aplastic anemia (SAA) and explore further the heterogeneous immunopathogenesis of SAA. The CD8(+)HLA-DR(+) cells were sorted by immunomagnetic separation from the PB of 24 untreated SAA patients and 23 normal controls. The mRNA expressions of perforin, granzyme B, FasL and tumor necrosis factor β (TNF-β) of sorted cells were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The mRNA levels of perforin, granzyme B of CD8(+)HLA-DR(+)T cells in untreated group were higher than those of the controls (0.66 ± 0.25, 0.56 ± 0.26 vs 0.53 ± 0.14, 0.40 ± 0.13, P = 0.042, 0.012). The mRNA level of FasL in CD8(+)HLA-DR(+) T cells of untreated SAA patients was higher than that of the controls (0.77 ± 0.24 vs 0.61 ± 0.16, P = 0.011). The mRNA of TNF-β in CD8(+)HLA-DR(+) T cells of untreated SAA patients was also higher than that of the controls (0.58 ± 0.16 vs 0.46 ± 0.15, P = 0.011). CD8(+)HLA-DR(+) T cells may damage hematopoiesis through the actions of perforin, granzyme B, TNF-β and FasL. And it thus contributes to the immunopathogenesis of SAA.