Synthesis of novel fluorescent 9,10-bisphenylethynylanthracene-derived pseudonucleoside and its introduction into oligonucleotides
Malakhov, A.D.; Prokhorenko, I.A.; Kuznetsova, S.V.; Skorobogatyĭ, M.V.; Korshun, V.A.; Berlin, I.A.
Bioorganicheskaia Khimiia 25(12): 933-937
1999
ISSN/ISBN: 0132-3423 PMID: 10734554 Document Number: 505993
The functionalization of the fluorescent hydrocarbon 9,10-bisphenylethynylanthracene to afford its dihydroxybutyl derivative was developed. Based on this pseudonucleoside, the phosphoramidite and a solid-phase carrier were obtained and used for the preparation of oligonucleotides containing a new fluorescent label for biopolymers.