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Use of a temporary protective group during the synthesis of oligonucleotides with an unnatural bond

Samoshin, V.V.; Kudiakov, I.E.; Smirnov, V.D.

Bioorganicheskaia Khimiia 17(2): 241-245

1991

ISSN/ISBN: 0132-3423
PMID: 1863283
Document Number: 371144
Methoxygroup has been used for transient P-protection on H-phosphonate oligonucleotide synthesis. Whereas other H-phosphonate linkages can be irreversibly transformed into phosphoramidates by treatment with alkylamines, the protected phosphoralkoxyl groups generate natural phosphodiester linkages after the final deprotection. The method allows for synthesis of oligonucleotides with addressed position of internucleotide phosphoralkylamidate groups.

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Semenov, A.N.; Lomonosova, I.V. 1993: Synthesis of a partially purified fragment of salmon calcitonin 1-16 using a phenylhydrazide protective group Bioorganicheskaia Khimiia 19(2): 182-189
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Cody, W.L.; Hruby, V.J. 1987: Unnatural amino acids: synthesis and biological significance Acta Universitatis Palackianae Olomucensis Facultatis Medicae 116: 495-516
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Schwartz, R.; Davis, R.; Hilton, T.J. 1992: Effect of temporary cements on the bond strength of a resin cement American Journal of Dentistry 5(3): 147-150
Batten, P.J. 1992: The descriptive epidemiology of unnatural deaths in Oregon's state institutions: a 25-year (1963-1987) study. III. A 25-year overview of unnatural deaths in the mental and correctional facilities American Journal of Forensic Medicine and Pathology 13(2): 159-168
Zarytova, V.F.; Ivanova, E.M.; Chasovskikh, M.N. 1990: Synthesis of steroid-containing oligonucleotides and their alkylating derivatives Bioorganicheskaia Khimiia 16(5): 610-616
Takeda, T.; Ikeda, K. 1983: Synthesis of oligonucleotides containing the hypermodified base, alpha-putrescinylthymine Nucleic Acids Symposium Series 12: 75-78
Antsypovich, S.I.; Volkov, E.M.; Oretskaia, T.S.; Romanova, E.A.; Tashlitskiĭ, V.N.; Blumenfeld, M.; Shabarova, Z.A. 1995: Synthesis of modified oligonucleotides and use of a duplex from them with covalently bound chains Bioorganicheskaia Khimiia 21(10): 774-780
Zhenodarova, S.M.; Khabarova, M.I. 1980: Stepwise synthesis of oligonucleotides. XXVIII. Synthetic activity of S-protein Molekuliarnaia Biologiia 14(2): 299-315
Malakhov, A.D.; Prokhorenko, I.A.; Kuznetsova, S.V.; Skorobogatyĭ, M.V.; Korshun, V.A.; Berlin, I.A. 1999: Synthesis of novel fluorescent 9,10-bisphenylethynylanthracene-derived pseudonucleoside and its introduction into oligonucleotides Bioorganicheskaia Khimiia 25(12): 933-937
Godovikova, T.S.; Zarytova, V.F.; Lokhov, S.G.; Mal'tseva, T.V.; Sergeev, D.S. 1990: Synthesis, structure and properties of rubomycin derivatives of mono- and oligonucleotides Bioorganicheskaia Khimiia 16(10): 1369-1378
Cech, D.; Schubert, F.; Möller, U. 1991: Non-radioactive labelling of oligonucleotides with pre-functionalized phosphoramidites or H-phosphonates during automated synthesis Nucleic Acids Symposium Series 24: 205-206
Chenna, A.; Maruenda, H.; Singer, B. 1999: Synthesis of para-benzoquinone and 1,3-bis(2-chloroethyl)nitrosourea adducts and their incorporation into oligonucleotides Iarc Scientific Publications 150: 89-101
Bokarewa, M.I.; Hellstrand, K.; Tarkowski, A. 2002: Phosphorotioated oligonucleotides trigger synthesis of human coagulation serine proteases Thrombosis and Haemostasis 88(5): 788-793
Ono, A.; Dan, A.; Matsuda, A. 1993: Synthesis of oligonucleotides carrying linker groups at 1'-position of sugar residues Nucleic Acids Symposium Series 29: 13-14