A new technic for counting Schistosoma japonicum eggs in pig feces
Willingham, A.L.; Johansen, M.V.; Barnes, E.H.
Southeast Asian Journal of Tropical Medicine and Public Health 29(1): 128-130
1998
ISSN/ISBN: 0125-1562 PMID: 9740285 Document Number: 491870
An improved laboratory method was developed for counting Schistosoma japonicum eggs in pig feces, which involves filtration, sedimentation and centrifugation, but avoids toxic chemicals. It is sensitive, allows easy differentiation from similar-sized and shaped protozoan cysts, and permits evaluation of egg viability both by direct viewing of eggs and miracidial hatching. It was found to be significantly better at recovering eggs than the modified Bell filtration technic. The sensitivity, specificity and practicality of this technic make it our method of choice for studies on porcine schistosomiasis japonica.