Effect of praziquantel on ATP uptake and metabolism of Schistosoma japonicum
Xiao, S.H.; Wang, C.Y.; Jiao, P.Y.; Chen, G.Z.; Yu, Y.G.; Yuan, X.J.
Zhongguo Yao Li Xue Bao 3(2): 130-135
1982
ISSN/ISBN: 0253-9756 PMID: 6214147 Document Number: 185996
When S. japonicum was exposed to culture medium containing [3H]ATP and praziquantel 0.1, 1 or 10 .mu.g/ml for 1-4 h, the [3H]ATP uptake decreased significantly in male worms, but not in females. If male schistosomes were exposed to praziquantel 1 or 10 .mu.g/ml for 1-4 h and then transferred to drug-free culture medium, the [3H]ATP uptake recovered to normal only in the lower drug concentration group. The [3H]ATP of male worms markedly decreased 1 h after intragastric gavage of praziquantel 100 mg/kg to infected mice and recovered to normal 4 h later. [3H]ATP uptake of male worms was steadily inhibited 24 h after 300 mg/kg to infected mice. No effect on [3H]ATP uptake of female worms was seen. Praziquantel promoted the transformation of [3H]ATP uptake by male worms to [3H]AMP, while the incorporation of [3H]ATP to RNA of male worms was markedly inhibited. No apparent changes were observed in female worms.