Partial purification of endogenous growth factor (s) of retinal pigment epithelial cells from neural retina
Katoh, K.; Ishiguro, S.; Tamai, M.
Nippon Ganka Gakkai Zasshi 100(2): 145-149
1996
ISSN/ISBN: 0029-0203 PMID: 8851154 Document Number: 467509
We attempted to purify endogenous growth factors of chick embryonal retinal pigment epithelial cells from bovine neural retina. Ammonium sulfate precipitation, gel filtration, and anion exchange column chromatography were used. Though only partially purification, bovine retinal extract had two effects, growth and transformation, on chick embryonal pigment epithelial cells. We think that these purified factors may be novel from the estimation of their molecular weight by gel filtration.