Tumor necrosis factor-alpha gene is expressed in stimulated retinal pigment epithelial cells in culture
Tanihara, H.; Yoshida, M.; Yoshimura, N.
Biochemical and Biophysical Research Communications 187(2): 1029-1034
1992
ISSN/ISBN: 0006-291X PMID: 1530600 Document Number: 389962
Expression of TNF-alpha gene in the retinal pigment epithelial (RPE) cells was studied by using polymerase chain reaction (PCR). PCR for human TNF-alpha gene using complementary DNA (cDNA) of control RNA prepared from non-stimulated RPE cells failed to show expression of TNF-alpha gene. However, PCR by using cDNA prepared from interleukin-1-beta (IL-1-beta)-stimulated RPE cells revealed expression of the gene, suggesting in vivo production of TNF-alpha from RPE cells in response to IL-1-beta.
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