NMR analysis of the backbone structure of DNA oligomers containing (5R) -/ (5S) -[5'-2H]-2'-deoxynucleosides
Ono, A.; Kubo, Y.; Makita, T.; Tate, S.; Kawashima, E.; Ishido, Y.; Kainosho, M.
Nucleic Acids Symposium Series 34: 195-196
1995
ISSN/ISBN: 0261-3166 PMID: 8841619 Document Number: 447757
In order to distinguish between NMR signals for pro-R and pro-S protons of the 5'-methylene group, DNA oligomers containing [5'-2H]-2'-deoxynucleosides were synthesized. As the deuterium labeled nucleosides were mixtures of [(5'S)-2H]- and [(5'R)-2H]-isotopomers approximately in a ratio of 2:1, the signals du to pro-R and pro-S protons were assigned undoubt-edly.