A differential scanning calorimetric-thin-layer chromatographic study of 5-halo-2'-deoxynucleosides
Olafsson, P.G.; Bryan, A.M.; Lau, K.
Journal of Chromatography 124(2): 388-393
1976
ISSN/ISBN: 0021-9673 PMID: 135000 Document Number: 101848
One of the difficulties in examining the influence of thermal stress on biologically active compounds via differential scanning calorimetry (DSC) has centered on the need for establishing the nature of the physical and/or chemical changes associated with the enthalpimetric transitions. Consequently this has tended to limit the study of thermal reactions via DSC to those substances for which the products have or can be separated on a macro scale. The ability to analyze DSC samples directly via TLC, following various segments of the heating cycle, removes this limitation. The applicability of this technique is demonstrated by an examination of the effects of heat on 5-halo-2'-deoxyuridines, and has shown that glycosidic cleavage occurs in each case.