Thin-layer chromatographic detection of glucocorticoids
Wu, S.M.; Chen, S.H.; Wu, H.L.
Gaoxiong Yi Xue Ke Xue Za Zhi 7(11): 545-549
1991
ISSN/ISBN: 0257-5655 PMID: 1766024 Document Number: 384349
An adsorption thin-layer chromatography (TLC) is described for the detection of glucocorticoids including triamcinolone, prednisolone, 6 alpha-methylprednisolone, betamethasone, dexamethasone, hydrocortisone, prednisone and cortisone. Separation of the glucocorticoids was performed on a silica stationary phase with chloroform-dioxane-methanol (15:4:1, v/v) as a mobile phase. The complete resolution of the epimers of betamethasone and dexamethasone was attained by derivatization of them with (1S)-(-)-camphanic chloride using triethylamine as a catalyst. The application of the method to the analysis of glucocorticoids in various samples is being smoothly developed.