Top Abstract Purchase Related
Home
>
Node
>
Subnode

Enzymatic synthesis of milligram quantities of ribozymes in small volumes

Hoffman, L.M.; Johnson, M.G.

Biotechniques 17(2): 372-375

1994

ISSN/ISBN: 0736-6205
PMID: 7526877
Document Number: 438321
Milligram quantities of ribozymes (Rzs) can be synthesized in vitro in reaction volumes of 1 mL or less using AmpliScribe T7 RNA polymerase kits to transcribe either linear plasmids or oligodeoxynucleotide templates. Model hammerhead Rzs were synthesized that specifically cleave RNA-encoding chloramphenicol acetyl transferase sequences. Methods are presented for the transcription of Rzs of virtually any length and sequence composition at a fraction of the cost of chemical synthesis.

Document emailed within 1 workday
Secure & encrypted payments

Kholmogorova, G.T.; Kostiukova, N.I. 1974: Isolation of immunoglobulin G from small quantities of human serum Laboratornoe Delo 10: 581-582
Evett, I.W.; Gill, P.D.; Jackson, G.; Whitaker, J.; Champod, C. 2002: Interpreting small quantities of DNA: the hierarchy of propositions and the use of Bayesian networks Journal of Forensic Sciences 47(3): 520-530
Ostrovsky, D.; Kharatian, E.; Malarova, I.; Shipanova, I.; Sibeldina, L.; Shashkov, A.; Tantsirev, G. 1992: Synthesis of a new organic pyrophosphate in large quantities is induced in some bacteria by oxidative stress Biofactors 3(4): 261-264
Monnat, R.J. 1979: Small volumes quantified Archives of Pathology and Laboratory Medicine 103(11): 602
Perrier, D.; Lear, E. 1976: Gas-chromatographic quantitation of theophylline in small volumes of plasma Clinical Chemistry 22(6): 898-900
Dybkaer, R. 1979: International Union of Pure and Applied Chemistry and International Federation of Clinical Chemistry. IUPAC Section on Clinical Chemistry Commission on Quantities and Units in Clinical Chemistry and IFCC Committee on Standards Expert Panel on Quantities and Units approved recommendation (1978). List of quantities in clinical chemistry Journal of Clinical Chemistry and Clinical Biochemistry 17(12): 822-835
Leonova, T.I.; Pressman, E.K. 1979: Determination of cholesterol and triglycerides in small volumes of blood serum Laboratornoe Delo 4: 244
Curran, D.P. 1998: Fluorous synthesis: an alternative to organic synthesis and solid phase synthesis for the preparation of small organic molecules Cancer Journal from Scientific American 4(Suppl 1): S73-S76
Skriabin, A.S.; Lazarev, V.N.; Zavgorodniaia, E.G. 1987: Determination of parameters of cellular immunity in small volumes of peripheral blood Laboratornoe Delo 4: 277-279
Kogan, B.M.; Nechaev, N.V. 1977: Spectrofluorometric method of determination of dopamine content in small blood volumes Laboratornoe Delo 12: 745-746
Cloyd, J.C.; Bosch, D.E.; Sawchuk, R.J. 1978: Concentration-time profile of phenytoin after admixture with small volumes of intravenous fluids American Journal of Hospital Pharmacy 35(1): 45-48
Killingsworth, C.R.; Slocombe, R.F.; Alnoor, S.A.; Robinson, N.E.; Derksen, F.J. 1987: Pulmonary dysfunction in neonatal calves after intratracheal inoculation of small volumes of fluid American Journal of Veterinary Research 48(11): 1589-1593
Himber, J.; Bühler, E.; Moll, D.; Moser, U.K. 1995: Low density lipoprotein for oxidation and metabolic studies. Isolation from small volumes of plasma using a tabletop ultracentrifuge International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 65(2): 137-142
Andrieux-Domont, C.; Le-van-Hung 1970: Influence of magnesium on enzymatic synthesis of coenzyme a Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 164(2): 292-296
Boccù, E.; Ebert, C.; Gardossi, L.; Gianferrara, T.; Zacchigna, M.; Linda, P. 1991: Enzymatic synthesis of ampicillin: a chemometric optimization Farmaco 46(4): 565-577
Sklyarenko, A.V.; EI'darov, M.A.; Kurochkina, V.B.; Yarotskii, S.V. 2015: Enzymatic Synthesis of Acidic β-Lactams (Review) Prikladnaia Biokhimiia i Mikrobiologiia 51(6): 546-560
Kurochkina, V.B.; Nys, P.S. 1999: Enzymatic synthesis of beta-lactam antibiotics. II. Aminocephalosporins Antibiotiki i Khimioterapiia 44(8): 6-11
Druzhinina, T.N.; Rozal'eva, V.V.; Shibaev, V.N.; Rozhnova, S.S.; Kilesso, V.A. 1986: Enzymatic synthesis of polyprenyl phosphosugars in Salmonella serotype C1 Bioorganicheskaia Khimiia 12(9): 1234-1239
Slomczynska, U.; Leplawy, T.; Leplawy, M.T. 1991: An efficient chemical-enzymatic synthesis of LHRH N-terminal pentapeptide Biomedica Biochimica Acta 50(10-11): S205-S208
Chernov, B.K.; Merits, A.; Mizenina, O.A.; Morozov, S.Iu. 1992: Chemical-enzymatic synthesis and cloning of the full-length genome of a DNA-containing plant virus Bioorganicheskaia Khimiia 18(12): 1487-1495