Enzymatic synthesis of polyprenyl phosphosugars in Salmonella serotype C1
Druzhinina, T.N.; Rozal'eva, V.V.; Shibaev, V.N.; Rozhnova, S.S.; Kilesso, V.A.
Bioorganicheskaia Khimiia 12(9): 1234-1239
1986
ISSN/ISBN: 0132-3423 PMID: 2430582 Document Number: 279880
The cell envelopes of serogroup C1 Salmonella, viz. S. thompson and S. montevideo, catalyze the transfer of radiolabeled sugars from UDP-[14C]Glc and UDP-[14C]GlcNAc into the lipid-linked sugars. Using TLC and DEAE-cellulose chromatography, the radiolabeled products were identified as polyprenyl pyrophosphate N-acetylglucosamine (I), polyprenyl monophosphate N-acetylglucosamine and polyprenyl monophosphate glucose. The derivative (I) served as an acceptor for mannose transfer from GDP-Man with formation of Man1-2GlcNAc1PPPre. A similar reaction was observed after addition of synthetic GlcNAc1PPPre to the cell envelopes.