A study of complement fixation test by trinitrophenyl-cap-liposome: application of viral antibody test
Yamauchi, T.; Yamamoto, Y.; Sawayanagi, Y.; Hukushima, M.; Hikizi, K.; Ueda, M.; Masaki, T.
Rinsho Byori. Japanese Journal of Clinical Pathology 42(7): 737-742
1994
ISSN/ISBN: 0047-1860 PMID: 8065041 Document Number: 434504
The complement fixation (CF) test is used as one of the diagnostic laboratory procedures for viral infections. The liposome is employed in the immunological method for basic research and we used the trinitrophenyl-cap-liposome (TNP-L) instead of sensitized erythrocyte for CF test. "TNP-L" consists of a plasma membrane of trinitrophenyl bases sensitized with antibody and is dissolved easily by animal complements. Carboxyfluorescein enclosed in liposome is released in fixed quantities according to liposome dissolution. Therefore, as our conclusion, the above CF method has a good agreement to that of sensitized erythrocyte. TNP-L method has advantages to be able to eliminate the individual differences and shorter storage-term of red blood cells and is also satisfactory for utilization with high reproducibility and intra day precision.