Antibody estimation by indirect complement fixation test for foot-and-mouth disease in cattle
Sakaki, K.; Suphavilai, P.; Tokuda, G.
National Institute of Animal Health Quarterly 17(2): 45-53
1977
ISSN/ISBN: 0027-951X PMID: 196222 Document Number: 118112
Serum samples were collected from cattle experimentally infected with FMD virus of O, A and Asia 1 types, as well as cattle infected in the field. Two types of antigen were used. One was by Frenkel's method with each type of the virus. The other was derived from the lingual epithelium of cattle infected by virus inoculation. Indirect complement fixation (ICF) antibody began to be detected about 4-5 days after inoculation. It reached a maximum titre 10-14 days after inoculation, remaining at this titre for about a week or two, and then decreased gradually. It was still detectable 63 days after inoculation. The rise and fall of ICF antibody was parallel with that of neutralizing antibody, although that antibody was always lower in titre than this. ICF antibody was detected type-specifically from cattle infected experimentally and naturally.