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Characterization of the plasmid pVS809 from Bifidobacterium globosum

Mattarelli, P.; Biavati, B.; Alessandrini, A.; Crociani, F.; Scardovi, V.

New Microbiologica 17(4): 327-331

1994

ISSN/ISBN: 1121-7138
PMID: 7861989
Document Number: 428721
A plasmid from a B. globosum strain was cut with 38 restriction enzymes and a physical map was constructed. Out of a total of 121 clones from curing experiments, plasmid was lost in 58% and 100% for acridine orange and ethidium bromide curing agent respectively. The plasmid does not exist as a chromosomal integrated form. An attempt to determine phenotypic characters encoded by the plasmid was made by electrophoretic analyses of the total proteins.

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