Determination of the optimal condition of hemoprotein immobilization on the polystyrene surface
Starodub, N.F.; Samokhina, L.M.; Rachkov, A.E.
Ukrainskii Biokhimicheskii Zhurnal 65(3): 17-22
1993
ISSN/ISBN: 0201-8470 PMID: 8291135 Document Number: 422484
The effect of pH, buffer composition, salts concentration and protein content on the immobilization efficiency of peroxidase, hemoglobin and their conjugates with bovine serum albumin (BSA) have been studied. All the substances were immobilized on the surface of polystyrene matrix. When using the 0.01 M carbonate-bicarbonate buffer (pH 9.6) immobilization of soluble substances is optimal. It was shown that the sorption efficiency of hemoproteins and their conjugates with BSA is considerably enhanced by drying solution containing ammonium bicarbonate. The increase of ammonium bicarbonate concentration from 0.13 M to 0.5 M leads to the process intensification.