Covalent immobilization of biomolecules onto polystyrene MicroWells for use in biospecific assays
Rasmussen, S.E.
Annales de Biologie Clinique 48(9): 647-650
1990
ISSN/ISBN: 0003-3898 PMID: 2099117 Document Number: 350983
Modification of polystyrene for higher binding capacity and/or for specific covalent immobilization of biomolecules is discussed. The benefit of covalent coupling of biomolecules onto a new commercially available surface type for covalent immobilization, CovaLink NH, is illustrated. The CovaLink NH solid phase has spacer arms covalently grafted onto the polystyrene solid phase, approximately 10(14) groups/cm2. Coupling procedures for covalent immobilization of biotin and peptides are demonstrated, and the advantage of using carbodiimide for coupling of carboxylic acid containing compounds is shown.