Covalent immunoglobulin assembly in vitro: reactivity of light chain covalent dimers (L2) and blocked light chain monomers
Kazin, A.R.; Beychok, S.
Science 199(4329): 688-690
1978
ISSN/ISBN: 0036-8075 PMID: 415360 Document Number: 135402
L2 and cysteine-blocked L chain monomers readily react with partially reduced H chains. A rapid disappearance of these blocked L chain species is followed by the appearance of covalent intermediates.sbd.HL, H2 and H2L.sbd.leading to the fully assembled H2L2. The mechanism of initial disulfide bond formation between H and L chains is disulfide interchange.
Document emailed within 1 workday
Related Documents
Buxbaum, J.N.; Chuba, J.V.; Hellman, G.C.; Solomon, A.; Gallo, G.R. 1990: Monoclonal immunoglobulin deposition disease: light chain and light and heavy chain deposition diseases and their relation to light chain amyloidosis. Clinical features, immunopathology, and molecular analysis Annals of Internal Medicine 112(6): 455-464Li, C.; Geng, H.; Yang, Z.; Zhong, R. 2011: Influence of Immunoglobulin Light Chain Dimers on the Results of the Quantitative Nephelometric Assay Clinical Laboratory 57(1-2): 53-57
Guloglu, F.Betul.; Smith, B.P.; Roman, C.A.J. 2008: Multiple levels of selection responsive to immunoglobulin light chain and heavy chain structures impede the development of Dmu-expressing B cells Journal of Immunology 181(6): 4098-4106
Levinson, S.S. 2010: Polyclonal free light chain of Ig may interfere with interpretation of monoclonal free light chain κ/λ ratio Annals of Clinical and Laboratory Science 40(4): 348-353
Sobczak, A.; Blazejczyk, M.; Piszczek, G.; Zhao, G.; Kuznicki, J.; Wojda, U. 2005: Calcium-binding calmyrin forms stable covalent dimers in vitro, but in vivo is found in monomeric form Acta Biochimica Polonica 52(2): 469-476
Lobb, C.J.; Olson, M.O.; Clem, L.W. 1984: Immunoglobulin light chain classes in a teleost fish Journal of Immunology 132(4): 1917-1923
Ščudla, V.; Pika, T.áš; Minařík, J.ří 2015: The role of the assessment of heavy/light chain pairs of immunoglobulin in monoclonal gammopathies Vnitrni Lekarstvi 61(1): 60
Clark, M.J.; Gagnon, J.; Williams, A.F.; Barclay, A.N. 1985: MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain EMBO Journal 4(1): 113-118
Perlmutter, R.M.; Briles, D.E.; Greve, J.M.; Davie, J.M. 1978: Light chain diversity of murine anti-streptococcal antibodies: IgCH-linked effects on L chain expression Journal of Immunology 121(1): 149-158
Sinclair, D.; Wainwright, L. 2007: How Lab Staff and the Estimation of Free Light Chains can Combine to Aid the Diagnosis of Light Chain Disease Clinical Laboratory 53(5-6): 267-271
Beishuizen, A.; Verhoeven, M.A.; Mol, E.J.; van Dongen, J.J. 1994: Detection of immunoglobulin kappa light-chain gene rearrangement patterns by Southern blot analysis Leukemia 8(12): 2228-36; discussion 2237-9
Gertz, M.A.; Dispenzieri, A. 2012: Immunoglobulin light-chain amyloidosis: growing recognition, new approaches to therapy, active clinical trials Oncology 26(2): 152-161
Delgado, F.; Kuus, K. 2014: Comparison of free κ and λ light chain immunoassays and total (bound and free) κ and λ light chain immunoassays Revista de Investigacion Clinica; Organo del Hospital de Enfermedades de la Nutricion 66(5): 473-474
Whicher, J.T.; Wallage, M.; Fifield, R. 1987: Use of immunoglobulin heavy- and light-chain measurements compared with existing techniques as a means of typing monoclonal immunoglobulins Clinical Chemistry 33(10): 1771-1773
Marchalonis, J.J.; Cone, R.E.; Rolley, R.T. 1973: Amplification of thymus-influenced lymphocytes by poly (A:U): inhibition of antigen binding by antiserum to immunoglobulin light chain Journal of Immunology 110(2): 561-566
Petrosian, M.N.; Vengerova, T.I.; Rokhlin, O.V. 1980: Immunochemical study of the conformational properties of the variable and constant domains of the light chain of mouse MOPC myeloma immunoglobulin Molekuliarnaia Biologiia 14(5): 1187-1192
Koelsch, R.; Lasch, J.; Hanson, H. 1970: Studies on covalent and non-covalent binding of leucine amino peptidase from bovine eye lenses to water insoluble carriers Acta Biologica et Medica Germanica 24(6): 833-846
VanNess, B.G.; Shapiro, M.; Kelley, D.E.; Perry, R.P.; Weigert, M.; D'Eustachio, P.; Ruddle, F. 1983: Aberrant rearrangement of the kappa light-chain locus involving the heavy-chain locus and chromosome 15 in a mouse plasmacytoma Nature 301(5899): 425-427
Mami, F.; Kindt, T.J. 1987: C lambda 2 and C lambda 4 immunoglobulin light chain genes in a wild-derived inbred mouse strain Journal of Immunology 138(11): 3980-3985
Adam, Z.; Stepánková, S.; Sirotková, A.; Cermáková, Z.; Pour, L.; Krejcí, M.; Zahradová, L.; Korístek, Z.; Lenz, J.; Hájek, R.; Vorlícek, J.; Mayer, J. 2011: Kidney failure in a patient with chronic B-lymphocytic leukaemia (B-CLL) with underlying cast nephropathy. the value of free immunoglobulin light chain identification for early diagnosis of this complication Vnitrni Lekarstvi 57(2): 214-221